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licl  (New England Biolabs)


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    New England Biolabs licl
    Licl, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 1506 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/licl/HiScribeT7+High+Yield+RNA+Syn+Kit/bio_rxiv__64898__2026__04__19__719519-143-16-17
    Average 99 stars, based on 1506 article reviews
    licl - by Bioz Stars, 2026-10
    99/100 stars

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    Related Articles

    Lysis:

    Article Title: Rapid diagnostic test using colorimetric LAMP
    Article Snippet: .. The lysis buffer was tested to determine an optimal formula for enhanced sensitivity of a LAMP assay for SARS-CoV-2. (a) A lysis buffer containing guanidine hydrochloride (GnHCL) (Millipore Sigma, Burlington, Mass.) was tested at various concentrations in the range of 10 mM-400 mM (1×) in combination with 1 mM, 4 mM and 8 mM Tris(2-carboxyethyl)phosphine hydrochloride (TCEP)(Millipore Sigma, Burlington, Mass.) (1×) with or without 75 mM LiCl (New England Biolabs, Ipswich, Mass. .. The lysis buffer was tested to determine an optimal formula for enhanced sensitivity of a LAMP assay for SARS-CoV-2. (a) A lysis buffer containing guanidine hydrochloride (GnHCL) (Millipore Sigma, Burlington, Mass.) was tested at various concentrations in the range of 10 mM-400 mM (1×) in combination with 1 mM, 4 mM and 8 mM Tris(2-carboxyethyl)phosphine hydrochloride (TCEP)(Millipore Sigma, Burlington, Mass.) (1×) with or without 75 mM LiCl (New England Biolabs, Ipswich, Mass.

    Article Title: Rapid diagnostic test using colorimetric lamp
    Article Snippet: .. The lysis buffer was tested to determine an optimal formula for enhanced sensitivity of a LAMP assay for SARS-CoV-2. (a) A lysis buffer containing guanidine hydrochloride (GnHCL) (Millipore Sigma, Burlington, Mass.) was tested at various concentrations in the range of 10 mM-400 mM (1×) in combination with 1 mM, 4 mM and 8 mM Tris(2-carboxyethyl)phosphine hydrochloride (TCEP)(Millipore Sigma, Burlington, Mass.) (1×) with or without 75 mM LiCl (New England Biolabs, Ipswich, Mass. .. 620151) (1×) against a SARS-CoV-2 virus titer that was varied for different samples containing 5,000 cps/ml, 10,000 cps/ml, 20,000 cps/ml or 40,000 cps/ml (from a stock solution of 100,000 cps/ml from SeraCare, Milford, Mass.).

    Article Title: Rapid diagnostic test for LAMP
    Article Snippet: .. The lysis buffer was tested to determine an optimal formula for enhanced sensitivity of a LAMP assay for SARS-CoV-2. (a) A lysis buffer containing guanidine hydrochloride (GnHCL) (Millipore Sigma, Burlington, Mass.) was tested at various concentrations in the range of 10 mM-400 mM (1×) in combination with 1 mM, 4 mM and 8 mM Tris(2-carboxyethyl)phosphine hydrochloride (TCEP)(Millipore Sigma, Burlington, Mass.) (1×) with or without 75 mM LiCl (NEB product B20151) (1×) against a SARS-CoV-2 virus titer that was varied for different samples containing 5,000 cps/ml, 10,000 cps/ml, 20,000 cps/ml or 40,000 cps/ml (from a stock solution of 100,000 cps/ml from SeraCare, Milford, Mass.). .. The lysis buffer was tested to determine an optimal formula for enhanced sensitivity of a LAMP assay for SARS-CoV-2. (a) A lysis buffer containing guanidine hydrochloride (GnHCL) (Millipore Sigma, Burlington, Mass.) was tested at various concentrations in the range of 10 mM-400 mM (1×) in combination with 1 mM, 4 mM and 8 mM Tris(2-carboxyethyl)phosphine hydrochloride (TCEP)(Millipore Sigma, Burlington, Mass.) (1×) with or without 75 mM LiCl (NEB product B20151) (1×) against a SARS-CoV-2 virus titer that was varied for different samples containing 5,000 cps/ml, 10,000 cps/ml, 20,000 cps/ml or 40,000 cps/ml (from a stock solution of 100,000 cps/ml from SeraCare, Milford, Mass.).

    Article Title: Rapid diagnostic test for LAMP
    Article Snippet: .. The lysis buffer was tested to determine an optimal formula for enhanced sensitivity of a LAMP assay for SARS-CoV-2. (a) A lysis buffer containing guanidine hydrochloride (GnHCL) (Millipore Sigma, Burlington, Mass.) was tested at various concentrations in the range of 10 mM-400 mM (1×) in combination with 1 mM, 4 mM and 8 mM Tris(2-carboxyethyl)phosphine hydrochloride (TCEP)(Millipore Sigma, Burlington, Mass.) (1×) with or without 75 mM LiCl (NEB product 620151) (1×) against a SARS-CoV-2 virus titer that was varied for different samples containing 5,000 cps/ml, 10,000 cps/ml, 20,000 cps/ml or 40,000 cps/ml (from a stock solution of 100,000 cps/ml from SeraCare, Milford, Mass.). .. The lysis buffer was tested to determine an optimal formula for enhanced sensitivity of a LAMP assay for SARS-CoV-2. (a) A lysis buffer containing guanidine hydrochloride (GnHCL) (Millipore Sigma, Burlington, Mass.) was tested at various concentrations in the range of 10 mM-400 mM (1×) in combination with 1 mM, 4 mM and 8 mM Tris(2-carboxyethyl)phosphine hydrochloride (TCEP)(Millipore Sigma, Burlington, Mass.) (1×) with or without 75 mM LiCl (NEB product 620151) (1×) against a SARS-CoV-2 virus titer that was varied for different samples containing 5,000 cps/ml, 10,000 cps/ml, 20,000 cps/ml or 40,000 cps/ml (from a stock solution of 100,000 cps/ml from SeraCare, Milford, Mass.).

    Lamp Assay:

    Article Title: Rapid diagnostic test using colorimetric LAMP
    Article Snippet: .. The lysis buffer was tested to determine an optimal formula for enhanced sensitivity of a LAMP assay for SARS-CoV-2. (a) A lysis buffer containing guanidine hydrochloride (GnHCL) (Millipore Sigma, Burlington, Mass.) was tested at various concentrations in the range of 10 mM-400 mM (1×) in combination with 1 mM, 4 mM and 8 mM Tris(2-carboxyethyl)phosphine hydrochloride (TCEP)(Millipore Sigma, Burlington, Mass.) (1×) with or without 75 mM LiCl (New England Biolabs, Ipswich, Mass. .. The lysis buffer was tested to determine an optimal formula for enhanced sensitivity of a LAMP assay for SARS-CoV-2. (a) A lysis buffer containing guanidine hydrochloride (GnHCL) (Millipore Sigma, Burlington, Mass.) was tested at various concentrations in the range of 10 mM-400 mM (1×) in combination with 1 mM, 4 mM and 8 mM Tris(2-carboxyethyl)phosphine hydrochloride (TCEP)(Millipore Sigma, Burlington, Mass.) (1×) with or without 75 mM LiCl (New England Biolabs, Ipswich, Mass.

    Article Title: Rapid diagnostic test using colorimetric lamp
    Article Snippet: .. The lysis buffer was tested to determine an optimal formula for enhanced sensitivity of a LAMP assay for SARS-CoV-2. (a) A lysis buffer containing guanidine hydrochloride (GnHCL) (Millipore Sigma, Burlington, Mass.) was tested at various concentrations in the range of 10 mM-400 mM (1×) in combination with 1 mM, 4 mM and 8 mM Tris(2-carboxyethyl)phosphine hydrochloride (TCEP)(Millipore Sigma, Burlington, Mass.) (1×) with or without 75 mM LiCl (New England Biolabs, Ipswich, Mass. .. 620151) (1×) against a SARS-CoV-2 virus titer that was varied for different samples containing 5,000 cps/ml, 10,000 cps/ml, 20,000 cps/ml or 40,000 cps/ml (from a stock solution of 100,000 cps/ml from SeraCare, Milford, Mass.).

    Article Title: Rapid diagnostic test for LAMP
    Article Snippet: .. The lysis buffer was tested to determine an optimal formula for enhanced sensitivity of a LAMP assay for SARS-CoV-2. (a) A lysis buffer containing guanidine hydrochloride (GnHCL) (Millipore Sigma, Burlington, Mass.) was tested at various concentrations in the range of 10 mM-400 mM (1×) in combination with 1 mM, 4 mM and 8 mM Tris(2-carboxyethyl)phosphine hydrochloride (TCEP)(Millipore Sigma, Burlington, Mass.) (1×) with or without 75 mM LiCl (NEB product B20151) (1×) against a SARS-CoV-2 virus titer that was varied for different samples containing 5,000 cps/ml, 10,000 cps/ml, 20,000 cps/ml or 40,000 cps/ml (from a stock solution of 100,000 cps/ml from SeraCare, Milford, Mass.). .. The lysis buffer was tested to determine an optimal formula for enhanced sensitivity of a LAMP assay for SARS-CoV-2. (a) A lysis buffer containing guanidine hydrochloride (GnHCL) (Millipore Sigma, Burlington, Mass.) was tested at various concentrations in the range of 10 mM-400 mM (1×) in combination with 1 mM, 4 mM and 8 mM Tris(2-carboxyethyl)phosphine hydrochloride (TCEP)(Millipore Sigma, Burlington, Mass.) (1×) with or without 75 mM LiCl (NEB product B20151) (1×) against a SARS-CoV-2 virus titer that was varied for different samples containing 5,000 cps/ml, 10,000 cps/ml, 20,000 cps/ml or 40,000 cps/ml (from a stock solution of 100,000 cps/ml from SeraCare, Milford, Mass.).

    Article Title: Rapid diagnostic test for LAMP
    Article Snippet: .. The lysis buffer was tested to determine an optimal formula for enhanced sensitivity of a LAMP assay for SARS-CoV-2. (a) A lysis buffer containing guanidine hydrochloride (GnHCL) (Millipore Sigma, Burlington, Mass.) was tested at various concentrations in the range of 10 mM-400 mM (1×) in combination with 1 mM, 4 mM and 8 mM Tris(2-carboxyethyl)phosphine hydrochloride (TCEP)(Millipore Sigma, Burlington, Mass.) (1×) with or without 75 mM LiCl (NEB product 620151) (1×) against a SARS-CoV-2 virus titer that was varied for different samples containing 5,000 cps/ml, 10,000 cps/ml, 20,000 cps/ml or 40,000 cps/ml (from a stock solution of 100,000 cps/ml from SeraCare, Milford, Mass.). .. The lysis buffer was tested to determine an optimal formula for enhanced sensitivity of a LAMP assay for SARS-CoV-2. (a) A lysis buffer containing guanidine hydrochloride (GnHCL) (Millipore Sigma, Burlington, Mass.) was tested at various concentrations in the range of 10 mM-400 mM (1×) in combination with 1 mM, 4 mM and 8 mM Tris(2-carboxyethyl)phosphine hydrochloride (TCEP)(Millipore Sigma, Burlington, Mass.) (1×) with or without 75 mM LiCl (NEB product 620151) (1×) against a SARS-CoV-2 virus titer that was varied for different samples containing 5,000 cps/ml, 10,000 cps/ml, 20,000 cps/ml or 40,000 cps/ml (from a stock solution of 100,000 cps/ml from SeraCare, Milford, Mass.).

    Virus:

    Article Title: Rapid diagnostic test for LAMP
    Article Snippet: .. The lysis buffer was tested to determine an optimal formula for enhanced sensitivity of a LAMP assay for SARS-CoV-2. (a) A lysis buffer containing guanidine hydrochloride (GnHCL) (Millipore Sigma, Burlington, Mass.) was tested at various concentrations in the range of 10 mM-400 mM (1×) in combination with 1 mM, 4 mM and 8 mM Tris(2-carboxyethyl)phosphine hydrochloride (TCEP)(Millipore Sigma, Burlington, Mass.) (1×) with or without 75 mM LiCl (NEB product B20151) (1×) against a SARS-CoV-2 virus titer that was varied for different samples containing 5,000 cps/ml, 10,000 cps/ml, 20,000 cps/ml or 40,000 cps/ml (from a stock solution of 100,000 cps/ml from SeraCare, Milford, Mass.). .. The lysis buffer was tested to determine an optimal formula for enhanced sensitivity of a LAMP assay for SARS-CoV-2. (a) A lysis buffer containing guanidine hydrochloride (GnHCL) (Millipore Sigma, Burlington, Mass.) was tested at various concentrations in the range of 10 mM-400 mM (1×) in combination with 1 mM, 4 mM and 8 mM Tris(2-carboxyethyl)phosphine hydrochloride (TCEP)(Millipore Sigma, Burlington, Mass.) (1×) with or without 75 mM LiCl (NEB product B20151) (1×) against a SARS-CoV-2 virus titer that was varied for different samples containing 5,000 cps/ml, 10,000 cps/ml, 20,000 cps/ml or 40,000 cps/ml (from a stock solution of 100,000 cps/ml from SeraCare, Milford, Mass.).

    Article Title: Rapid diagnostic test for LAMP
    Article Snippet: .. The lysis buffer was tested to determine an optimal formula for enhanced sensitivity of a LAMP assay for SARS-CoV-2. (a) A lysis buffer containing guanidine hydrochloride (GnHCL) (Millipore Sigma, Burlington, Mass.) was tested at various concentrations in the range of 10 mM-400 mM (1×) in combination with 1 mM, 4 mM and 8 mM Tris(2-carboxyethyl)phosphine hydrochloride (TCEP)(Millipore Sigma, Burlington, Mass.) (1×) with or without 75 mM LiCl (NEB product 620151) (1×) against a SARS-CoV-2 virus titer that was varied for different samples containing 5,000 cps/ml, 10,000 cps/ml, 20,000 cps/ml or 40,000 cps/ml (from a stock solution of 100,000 cps/ml from SeraCare, Milford, Mass.). .. The lysis buffer was tested to determine an optimal formula for enhanced sensitivity of a LAMP assay for SARS-CoV-2. (a) A lysis buffer containing guanidine hydrochloride (GnHCL) (Millipore Sigma, Burlington, Mass.) was tested at various concentrations in the range of 10 mM-400 mM (1×) in combination with 1 mM, 4 mM and 8 mM Tris(2-carboxyethyl)phosphine hydrochloride (TCEP)(Millipore Sigma, Burlington, Mass.) (1×) with or without 75 mM LiCl (NEB product 620151) (1×) against a SARS-CoV-2 virus titer that was varied for different samples containing 5,000 cps/ml, 10,000 cps/ml, 20,000 cps/ml or 40,000 cps/ml (from a stock solution of 100,000 cps/ml from SeraCare, Milford, Mass.).

    Plasmid Preparation:

    Article Title: Comparative Kinetic Analysis of OXA-438 with Related OXA-48-Type Carbapenem-Hydrolyzing Class D β-Lactamases.
    Article Snippet: DNA restriction was done with HindIII and PstI [New England Biolabs (NEB), Ipswich, MA, USA]. .. After restriction, plasmid DNA was precipitated with LiCl and ligated with T4 DNA Ligase (NEB, Ipswich, MA, USA). pACyC and lambda phage DNA were used as controls. ..



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    Image Search Results


    (A) Left, cryo-EM map of the FEX-CA/10E8v4 Fab complex. Right, atomic model of FEX-CA/10E8v4 Fab complex. For both panels, FEX-CA is shown in yellow, the 10E8v4 Fab heavy chain in salmon pink, and the light chain in green. Dashed lines indicate the approximate boundaries of the plasma membrane, with the cytoplasmic (in) and extracellular (out) sides labeled. (B) Structural comparison of FEX-CA in the presence of NaCl and LiCl analyzed using Resi-Ruler . Domain 1 of the Na⁺ and Li⁺ structures are superimposed and the magnitude of the per-residue C α distance differences is displayed as a color gradient (purple, Na + -FEX-CA; orange, Li + -FEX-CA). (C-D). Cross-sectional views of FEX-CA structures determined with Li⁺ (top) or Na⁺ (bottom). Cavities of the functional pore are shown from the extracellular view (C) or side (D), with cross-sectional regions at the same level, as indicated by yellow dashed lines. Protein surfaces of FEX-CA are colored by electrostatic potential as shown in the scale.

    Journal: bioRxiv

    Article Title: The structural mechanism of eukaryotic fluoride channel activation and inhibition by monovalent cations

    doi: 10.64898/2026.04.21.719972

    Figure Lengend Snippet: (A) Left, cryo-EM map of the FEX-CA/10E8v4 Fab complex. Right, atomic model of FEX-CA/10E8v4 Fab complex. For both panels, FEX-CA is shown in yellow, the 10E8v4 Fab heavy chain in salmon pink, and the light chain in green. Dashed lines indicate the approximate boundaries of the plasma membrane, with the cytoplasmic (in) and extracellular (out) sides labeled. (B) Structural comparison of FEX-CA in the presence of NaCl and LiCl analyzed using Resi-Ruler . Domain 1 of the Na⁺ and Li⁺ structures are superimposed and the magnitude of the per-residue C α distance differences is displayed as a color gradient (purple, Na + -FEX-CA; orange, Li + -FEX-CA). (C-D). Cross-sectional views of FEX-CA structures determined with Li⁺ (top) or Na⁺ (bottom). Cavities of the functional pore are shown from the extracellular view (C) or side (D), with cross-sectional regions at the same level, as indicated by yellow dashed lines. Protein surfaces of FEX-CA are colored by electrostatic potential as shown in the scale.

    Article Snippet: Lysate was extracted with 2% n-Dodecyl-β-D-Maltoside (DDM) (Anatrace, Maumee, OH) for 2 hours at room temperature and protein purified using Strep-Tactin XT 4Flow resin (IBA Lifescience, Pittsburgh, PA) equilibrated with wash buffer (20 mM Tris-HCl, pH 8.0, 100 mM NaCl, 5 mM NaF, 1 mM DDM) followed by size-exclusion chromatography (SEC) (Superdex 200, Cytiva, Marlborough, MA) in SEC buffer (20 mM 4-(2-Hydroxyethyl)piperazine-1-ethanesulfonic acid (HEPES) pH 7.5, 150 mM LiCl, 5 mM NaF, and 0.5 mM DDM for cryo-EM or 1 mM DDM for functional studies.

    Techniques: Cryo-EM Sample Prep, Clinical Proteomics, Membrane, Labeling, Comparison, Residue, Functional Assay

    (A) Size exclusion chromatograms of MPER-tagged FEX-CA alone (gray dashed line) or with 10E8v4 Fab at a 1:1 molar ratio (blue trace) in the presence of 150 mM LiCl. Inset: Coomassie-stained SDS-PAGE of the peak fraction corresponding to the FEX-CA/10E8v4 Fab complex. (B) Representative cryo-EM micrograph and 2D averages of the FEX-CA/10E8v4 Fab complex in DDM micelles, with a 100 Å scale bar indicated. (C) Fourier shell correlation (FSC) curves as a function of resolution calculated between the half maps. (D) Local resolution map colored by local resolution estimate (B factor -137.8) using cryoSPARC. (E) Particle angular distribution plot.

    Journal: bioRxiv

    Article Title: The structural mechanism of eukaryotic fluoride channel activation and inhibition by monovalent cations

    doi: 10.64898/2026.04.21.719972

    Figure Lengend Snippet: (A) Size exclusion chromatograms of MPER-tagged FEX-CA alone (gray dashed line) or with 10E8v4 Fab at a 1:1 molar ratio (blue trace) in the presence of 150 mM LiCl. Inset: Coomassie-stained SDS-PAGE of the peak fraction corresponding to the FEX-CA/10E8v4 Fab complex. (B) Representative cryo-EM micrograph and 2D averages of the FEX-CA/10E8v4 Fab complex in DDM micelles, with a 100 Å scale bar indicated. (C) Fourier shell correlation (FSC) curves as a function of resolution calculated between the half maps. (D) Local resolution map colored by local resolution estimate (B factor -137.8) using cryoSPARC. (E) Particle angular distribution plot.

    Article Snippet: Lysate was extracted with 2% n-Dodecyl-β-D-Maltoside (DDM) (Anatrace, Maumee, OH) for 2 hours at room temperature and protein purified using Strep-Tactin XT 4Flow resin (IBA Lifescience, Pittsburgh, PA) equilibrated with wash buffer (20 mM Tris-HCl, pH 8.0, 100 mM NaCl, 5 mM NaF, 1 mM DDM) followed by size-exclusion chromatography (SEC) (Superdex 200, Cytiva, Marlborough, MA) in SEC buffer (20 mM 4-(2-Hydroxyethyl)piperazine-1-ethanesulfonic acid (HEPES) pH 7.5, 150 mM LiCl, 5 mM NaF, and 0.5 mM DDM for cryo-EM or 1 mM DDM for functional studies.

    Techniques: Staining, SDS Page, Cryo-EM Sample Prep